Process HPLC Column, RP C4, Wide Pore, 10um, 300A. 50mm ID x 100mm Length. Cogent Brand. 1 EA.

Additional Information:

Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. The 300 Å pore structure is specifically designed to improve accessibility for proteins, peptides, enzymes, and other large biomolecules, making it an excellent choice for process-scale purification applications where high recovery, loading capacity, and reproducible performance are critical.

68004-P50-1003M is a process HPLC column with dimensions of 50 mm ID x 100 mm length, packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The large internal diameter is designed for substantial sample loading and high-throughput purification, while the 100 mm length provides an effective balance between productivity and chromatographic resolution. Typical flow rates are approximately 80–200 mL/min depending on system capabilities, mobile phase conditions, and process requirements.

This column is specifically intended for in-process purification of proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. The combination of wide pore architecture, fully endcapped C4 chemistry, and process-scale dimensions provides excellent analyte recovery and efficient purification performance. Applications include biopharmaceutical production support, biotechnology research, process development, manufacturing scale-up, and biomolecule production workflows. A specialized process-scale HPLC system capable of high flow rates, large injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.

Key Benefits

  • RP C4 Butyl Wide Pore stationary phase

  • 300 Å pore size optimized for biomolecules

  • Fully double endcapped chemistry

  • Traditional butyl (C4) bonded phase

  • Designed for process-scale purification

  • Excellent recovery of proteins and peptides

  • High sample loading capacity

  • 10 µm particles for lower operating backpressure

  • Suitable for high flow rates

  • Balanced throughput and resolution with 100 mm length

Typical Applications

  • Process-scale biomolecule purification

  • Protein purification

  • Peptide purification

  • Enzyme purification

  • Biopharmaceutical production support

  • Biotechnology research

  • Process development

  • Manufacturing scale-up

  • Production workflows

  • Fraction collection

Specifications

  • Product Type: Process HPLC Column

  • Stationary Phase: RP C4 Butyl Wide Pore

  • Chromatography Mode: Reversed Phase

  • Bonded Phase: Butyl (C4)

  • Endcapping: Fully Double Endcapped

  • Particle Size: 10 µm

  • Pore Size: 300 Å

  • Column Dimensions: 50 mm ID x 100 mm

  • Length: 100 mm

  • Typical Flow Rate: 80–200 mL/min

  • Brand: Cogent

Special Order Item Information

Important Notes

  • Designed for purification rather than routine analytical HPLC

  • Intended for collection and isolation of purified biomolecules

  • Optimized for proteins, peptides, enzymes, and other large molecules

  • Requires a process-scale HPLC system capable of high flow rates and fraction collection

  • Wide pore structure improves accessibility and recovery of biomolecules

  • Suitable for process development, manufacturing scale-up, and production-support purification

  • Special Order Item


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